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KMID : 0363620060270020122
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Journal of Korean Oriental Medicine
2006 Volume.27 No. 2 p.122 ~ p.123
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Protective Effects of Hemerocallis Fulva Extracts on Amyloid beta-Protein-Induced Death in Neuronal Cells
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Kim Eun-Suk
Choi Soo-Jin
Ryu Beung-Ho
Choi Jin-Ho
Oh Myung-Suk
Park Woo-Jin
Choi Young-Hwan
Paik Do-Hyeon
Ha Kwon-Chul
Kang Dae-Won
Cho Yong-Kweon
Park Ki-Tae
Moon Ja-Young
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Abstract
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Objectives :The amyloid beta-protein (Abeta) is the principal component of the senile plaques characteristic of Alzheimer’’s disease (AD) and elicits a toxic effect on neurons in vitro and in vivo. Many environmental factors including antioxidants and proteoglycans modify A{beta}toxicity. In this study, we have investigated the protective effects of water- and organic solvent-extracts of Hemerocallis fulva root fractions pre-extracted with methanol on Abeta-induced oxidative cell death in cultured rat pheochromocytoma (PC12) cells.
Methods :For this study, we used MTT reduction assay for detection of protective effects of water- and organic solvent-extracts of Hemerocallis fulva root fractions pre-extracted with methanol on A{beta}_{25-35}-induced cytotoxicity to PC12 cells. We also used cell-based beta-secretase assay system to investigate the inhibitory effect of water- and organic solvent-extracts of Hemerocallis fulva root on beta-secretase activity.
Results :We previously reported that methanol extracts of Hemerocallis fulva root strongly attenuated cytotoxicity induced by the three Abeta fragments (A{beta}_{25-35},;A{beta}_{1-42};A{beta}_{1-43}) to both SK-N-MC and PC12 cells. In the present study, we found that butanol-, ethylacetate-, chloroform-, and water-extracts of Hemerocallis fulva root fractions pre-extracted with methanol had strong protective effects against A{beta}_{25-35}-induced cytotoxicity to PC12 cells and inhibitory potency to beta-secretase activity.
Conclusion :These results suggest that butanol-, ethylacetate-, chloroform-, and water-extracts of Hemerocallis fulva root fractions pre-extracted with methanol may contain the protective component(s) against Abeta-induced cell death in PC12 cells as well as inhibitory component(s) to beta-secretase activity.
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KEYWORD
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amyloid beta-protein, BACE, beta-secretase, Hemerocallis fulva, Liliaceae, PC12 cell
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